Association of IDH1 Gene R132H Mutation and Increased Tumor HIF1‐α and Serum VEGF Levels in Primary Glioblastoma Multiforme
Makaleler > Tam Makale · 2015
Yazarlar: Hikmet Hakan AYDIN, Cem Yalaza, Handan Ak, Mehmet Cagli, Erkin ÖZGİRAY, Sevcan Atay
The FASEB Journal, Uluslararası, Wos, 2015, cilt 29
DOI: 10.1096/fasebj.29.1_supplement.725.18
Özet
Glioblastoma multiforme (GBM) is the most common form of primary brain tumors. Although mutations in isocitrate dehydrogenase‐1 ( IDH1 ) have been identified in a number of cancers, their role in tumor development has not been fully elucidated. In this preliminary study we aimed to investigate the association between IDH1 mutations, tumor tissue HIF‐1 alpha and serum VEGF levels in patients with primary GBM. 32 patients (mean age, years: 58 ± 14.0) diagnosed with primary glioblastoma multiforme were screened for IDH1 mutations (R132H, R132S, R132C and R132L) by direct sequencing. Serum VEGF and tumor tissue HIF1‐alpha levels were measured by ELISA. Associations between categoric variables were determined using chi‐square tests. Differences between two groups were compared with t test for continuous variables. Two patients were heterozygous for R132H mutation (6%). Tumor HIF1‐alpha and serum VEGF levels were found to be significantly increased in IDH1 ‐mutated tumor tissues. Wild Type IDH1 (n=30) Mutant IDH1 (n=2) p Serum VEGF (ng/ml) 0.189 ± 0.08 0.309 ± 0.016 0.0454 Tumor HIF1‐α (ng/ml) 0.710 ± 0.835 5.058 ± 3.75 < 0.0001 Our preliminary results suggest that mutated IDH1 may contributes to carcinogenesis via induction of HIF‐1 alpha pathway in primary GBM.