A novel DAD type and folic acid conjugated fluorescent monomer as a targeting probe for imaging of folate receptor overexpressed cells
Articles > Article ยท 2014
Authors: Fulya Ekiz Kanik, Didem Ag, Muharrem Seleci, Firat Baris Barlas, Melis Kesik, Gonul Hizalan, Hava Akpinar, Suna Timur, Levent Toppare
Biotechnology Progress, International, WoS, 2014, vol 30, pp 952-959
DOI: 10.1002/btpr.1928
Summary
We describe a modification and post-functionalization technique for a donor-acceptor-donor type monomer; 6-(4,7-bis(2,3-dihydrothieno[3,4-b][1,4]dioxin-5-yl)-2H-benzo[d][1,2, 3]triazol-2-yl)hexan-1-amine. Folic acid was attached to the fluorescent structure. The conjugation was confirmed via NMR and Fourier transform infrared analyses. Cytotoxicity was investigated and the comparison of association of targeted monomeric structures in tumor cells was monitored via fluorescence microscopy.