COMPARISON OF MICROTISSUE FORMING CAPACITY OF SH-SY5Y AND SK-N-AS CELL LINES
Makaleler > Tam Makale · 2016
Yazarlar: Aslı Aybike DOĞAN, ŞEYMA TAŞDEMİR, Aylin ŞENDEMİR
Deu Muhendislik Fakultesi Fen ve Muhendislik, Diğer Kurumların Hakemli Dergileri, Uluslararası, TR Dizin (Ulakbim), 2016, cilt 18, ss 40-40
DOI: 10.21205/deufmd.20165217544
Özet
Two-dimensional (2D) cell culture systems are important tools for basic in vitro research.However, they form a thin monolayer structure and poorly mimic the complex in vivo conditions in terms of biochemical signals, cell-cell and cell-extracellular matrix (ECM) interactions.In this study, we performed a comparative study on SH-SY5Y and SK-N-AS neuroblastoma cell lines in terms of their microtissue forming capacity.Both cell lines are commonly used to model neurodegenerative diseases in vitro.Cells were cultured using 3D Petri Dish ® technique.The cells' microtissue forming capacity was observed morphologically and microtissues' size was analized.Results indicate that microtissue forming capacity of SH-SY5Ycell line was better than that of SK-N-AS cell line.SH-SY5Y microtissues can be used as an alternative, scaffold-free in vitro 3D model for neurodegenerative diseases and neuroblastoma research.