Quercetin-induced apoptosis involves increased hTERT enzyme activity of leukemic cells
Makaleler > Tam Makale · 2011
Yazarlar: Çığır BİRAY AVCI, Sunde YILMAZ SÜSLÜER, Zeynep Ozlem Dogan, Güray SAYDAM, Yavuz Dodurga, Huseyin Atakan Ekiz, Melis Kartal, Fahri ŞAHİN, Yusuf Baran, Cumhur GÜNDÜZ
HEMATOLOGY, SCI Expanded İndekslerine Giren Dergi, Uluslararası, 2011, cilt 16, ss 303-307
DOI: 10.1179/102453311x13085644680104
Özet
We aimed to examine the growth suppressive effects of quercetin on acute promyelocytic and lymphoblastic leukemia and chronic myeloid leukemia, and to find out whether the growth suppression is related to the blocking of telomerase enzyme activity. Cytotoxic effects of quercetin were shown by trypan blue analyses. Apoptotic effects of quercetin were examined by acridine orange and ethidium bromide staining by fluorescence microscopy. The effects of quercetin on telomerase enzyme activity were shown by hTERT Quantification Kit. Our results demonstrated that quercetin has antiproliferative and apoptotic effects on T-cell acute lymphoblastic leukemia (ALL), acute promyelocytic leukemia, and chronic myeloid leukemia (CML) cells. We also showed for the first time by this study that quercetin suppresses the activity of telomerase in ALL and CML cells. The results of this study show the importance of quercetin for its therapeutic potential in treatment of leukemias.