Quantitative Determination of Galanthamine and Lycorine in an endemic Galanthus species: G. cilicicus
Makaleler > Tam Makale · 2011
Yazarlar: Gülen İrem KAYA, D Cicek Polat, MA Onur, Nehir SOMER
Planta Medica, Uluslararası, Wos, 2011, cilt 77
Özet
Galanthus cilicicus Baker, an endemic species of the genus Galanthus L. (Amaryllidaceae), is distributed in southern Turkey mainly in the province of Içel [1]. Galanthamine, the most important alkaloid found in Amaryllidaceae species, is used for the treatment of Alzheimer's disease [2]. Lycorine, another important and also a widespread alkaloid found in Amaryllidaceae plants has been proven to have several biological activities [3,4]. A reversed-phase high-performance liquid chromatographic method has been used and validated for the determination of lycorine and galanthamine in G. cilicicus . The extraction of both alkaloids in low-mass plant samples, were carried out by a simple and a rapid method utilizing pre-packed columns with diatomaceous earth (Extrelut ® ) [5]. The chromatographic separation was performed using an isocratic system with a mobil phase of trifluoroacetic acid-water-acetonitrile (0.01: 92.5: 7.5) and diode array dedector [6]. The linearity of the method was studied by injecting five known concentrations of lycorine in the range of 1–10µg mL -1 and five known concentrations of galanthamine in the range of 2.5–20µg mL -1 The calibration curves for lycorine and galanthamine were determined as Y=13.2828995x + 0.4488635 and Y=10.1354031x +0.5465348, respectively. Validation procedures showed that the method was specific, accurate and precise. The above-mentioned method was applied to the aerial parts and bulbs of G.cilicicus . The contents of galanthamine and lycorine in the bulbs of G.cilicicus were found to be 0.016% and 0.0035%, respectively. In the aerial parts, lycorine was not detected, however the content of galanthamine was found as 0.015%. Acknowledgement: This study was financially supported by Ege University Research Fund (09/ECZ/037) and partially supported by TUBITAK (TBAG-104T272) and EBILTEM (2007-BIL-007). References: 1. Davis AP (2006) The Genus Galanthus-Snowdrops in the Wild, in Bishop M., Davis A.P., Grimshaw J. (Eds.), Snowdrops, A Monograph of Cultivated Galanthus. Griffin Press Publishing Ltd. Cheltenham. 2. Heinrich M, Teoh HL (2004)J Ethnopharmacol 92:147–162. 3. Szlàvik L, et al. (2004) Planta Med 70: 871–873. 4. Sener B et al. (2003) Phytotherapy Res 17: 1220–1223. 5. Berkov S et al. (2008) Phytochem Anal 19: 285–293. 6. Mustafa NR et al. (2003)J Liq Chromatogr R T 26: 3217–3233.