Optical Determination of Glucose with Glucose Oxidase Immobilized in PVC together with Fluorescent Oxazol‐5‐One Derivatives
Makaleler > Tam Makale · 2008
Yazarlar: Gulsiye Ozturk, Suna Timur, Serap Alp
Analytical Letters, Uluslararası, Wos, 2008, cilt 41, ss 608-618
DOI: 10.1080/00032710801910551
Özet
Fully reversible biosensors for glucose monitoring based on solid polyvinylchloride (PVC) films where the enzyme glucose oxidase (GOx) was incorporated together with 2‐phenyl‐4‐[4‐(1,4,7,10‐tetraoxa‐13‐azacyclopentadecyl)benzylidene]‐5‐oxazolone (CPO‐1), 2‐(3,5‐dinitrophenyl)‐4‐[4‐(1,4,7,10‐tetraoxa‐13‐azacyclopentadecyl)benzylidene]‐5‐oxazolone (CPO‐2), 2‐(4‐nitrophenyl)‐4‐[4‐(1,4,7,10‐tetraoxa‐13‐azacyclopentadecyl)benzylidene]‐5‐oxazolone (CPO‐3) and 2‐(4‐tolyl)‐4‐[4‐(1,4,7,10‐tetraoxa‐13‐azacyclopentadecyl)benzylidene]‐5‐oxazolone (CPO‐4) derivatives have been developed. The limit of detection (LOD) values for glucose were found to be 1.47×10−5 M, 2.01×10−5 M, 0.89×10−5 M, and 0.12×10−5 M for CPO‐1, CPO‐2, CPO‐3, and CPO‐4, respectively (n=7). Sensor films were found to have excellent photostability.