Development and Evaluation of Liquid and Solid Lipid Based Drug Delivery Systems Containing Technetium-99m-Radiolabeled Alendronate Sodium
Makaleler > Tam Makale · 2018
Yazarlar: Evren ATLIHAN GÜNDOĞDU, Meliha EKİNCİ, Emre ÖZGENÇ, Derya Ozdemir, MAKBULE AŞIKOĞLU
CURRENT RADIOPHARMACEUTICALS, SCI Expanded İndekslerine Giren Dergi, Uluslararası, 2018, cilt 11, ss 100-108
DOI: 10.2174/1874471011666180529111914
Özet
<h3 style="font-size: 1.0769em; line-height: 1.2857; margin: 0px; color: rgb(152, 87, 53); font-weight: bold; display: inline; font-family: arial, helvetica, clean, sans-serif; letter-spacing: normal;">Abstract</h3><div class="" style="font-family: arial, helvetica, clean, sans-serif;"><h4 style="font-size: 1em; margin: 0px 0.25em 0px 0px; color: rgb(0, 0, 0); font-weight: bold; text-transform: uppercase; float: left;">OBJECTIVE:</h4><p style="margin-bottom: 0.5em; font-size: 1.04em;">The purpose of this study was to develop lipid-water based drug delivery system of Alendronate Sodium (ALD) in liquid and solid form obtained by using spray drying method and compare these two forms with radioactive cell culture studies.</p><h4 style="font-size: 1em; margin: 0px 0.25em 0px 0px; color: rgb(0, 0, 0); font-weight: bold; text-transform: uppercase; float: left;">METHODS:</h4><p style="margin-bottom: 0.5em; font-size: 1.04em;">This study included the development of liquid and solid form obtained by spray drying method, radiolabelling of ALD with 99mTc, preparation of formulations containing 99mTc -ALD and evaluation of their permeability with Caco-2 cell. The liquid formulations have been developed by using various surfactants, co-surfactants, oil and water phases. Physicochemical characterizations like droplet size, polydispersity index (PDI) and zeta potential measurements and short term stability studies were investigated.</p><h4 style="font-size: 1em; margin: 0px 0.25em 0px 0px; color: rgb(0, 0, 0); font-weight: bold; text-transform: uppercase; float: left;">RESULTS:</h4><p style="margin-bottom: 0.5em; font-size: 1.04em;">According to the measurement results, two oil in water formulations (F1-L and F2-L) were selected and spray dried with Buchi mini spray dryer apparatus to provide solid formulations (F1-S and F2-S). ALD was labeled with 99mTc and added to formulations. The effect of experimental conditions on radiolabeling efficiency of ALD and stability of all formulations containing 99mTc-ALD were investigated through Radio Thin Layer Chromatography (RTLC). It was observed that the labeling efficiency of ALD was greater than 90% and all formulations were found to be stable up to 6 h at room temperature. Permeability of radiolabeled ALD from all formulations was performed by using Caco-2 cells. According to the cell culture studies, permeability from spray dried formulations of ALD was found higher than liquid formulations.</p><h4 style="font-size: 1em; margin: 0px 0.25em 0px 0px; color: rgb(0, 0, 0); font-weight: bold; text-transform: uppercase; float: left;">CONCLUSION:</h4><p style="margin-bottom: 0.5em; font-size: 1.04em;">As a conclusion, spray dried formulations could be a promising drug delivery system for enhancing the permeability of ALD. Furthermore, this study is a good example of the use of radiolabeled compounds in drug development.</p><h4 style="font-size: 11.999px; margin: 0px 0.35em 0px 0px; color: rgb(0, 0, 0); font-weight: bold; text-transform: uppercase; float: left; font-family: arial, helvetica, clean, sans-serif;">KEYWORDS:</h4><p style="margin-top: 0.5em; margin-bottom: 0.5em; font-size: 11.999px;">RTLS; Spray drying method; alendronate sodium; lipid-water based drug delivery system; radiolabeling; technetium-99m.</p></div>