Effects of Hyperbaric Oxygen Therapy on the Morphological Characteristics and Survival of MCF-7 Breast Cancer Cells
Articles > Article · 2023
Authors: GÜRKAN YİĞİTTÜRK, SERKAN ERGÖZEN, HÜLYA ELBE, ANIL YÜCEL, TÜRKER ÇAVUŞOĞLU, TUBA BAYGAR, Yiğit UYANIKGİL
Ege Tıp dergisi, Refereed Journals of Other Institutions, International, TR DİZİN (ULAKBİM), 2023, vol 62, pp 12-17
DOI: 10.19161/etd.1235892
Summary
Aim: This study aims to determine the effects of hyperbaric oxygen therapy at different pressure values on cell morphology and cell survival in the MCF-7 breast cancer cell line. Materials and Methods: The experimental groups were formed by applying 100% oxygen to MCF-7 breast cancer cells at 1.5, 2, and 2.5 atmospheres for 2 hours. The control group did not receive treatment. At the end of the experiment, cell survival was investigated by CCK-8 analysis, cell shapes were determined by cresyl violet staining, and cell surface morphologies were determined by scanning electron microscope. Results: Cell viability was significantly reduced at atmospheric pressure of 1.5, 2, and 2.5 compared to the control group (p < 0.005). As pressure increased, the surface area of the cell decreased, nuclear condensation increased, and the cell borders became irregular. Cell membrane bleb and cell membrane porosity increased at 2 and 2.5 atmospheres. Conclusion: Hyperbaric oxygen therapy severely reduces the viability of MCF-7 breast cancer cells under increased pressure. It can induce apoptosis and change the shape and surface morphology of MCF-7 breast cancer cells. Although further studies are needed, our study supports the potential use of hyperbaric oxygen therapy in the treatment of breast cancer.