The Effect of Freezing on the Immunoprofile of Breast Carcinoma Cells
Makaleler > Tam Makale · 2014
Yazarlar: ASUMAN ARGON, A ŞENER, Osman ZEKİOĞLU, M KAPKAÇ, Necmettin ÖZDEMİR
Balkan Medical Journal, SCI Expanded İndekslerine Giren Dergi, Uluslararası, Wos, 2014, cilt 31, ss 335-339
DOI: 10.5152/balkanmedj.2014.14389
Özet
Breast cancer is the most common cancer in women (1).Therapeutic developments and advances in public health services increasing awareness regarding early diagnosis are rapidly progressing.Although surgery is the primary treatment in early stage breast cancers, neoadjuvant and adjuvant treatments are also important for local advanced and metastatic patients (2).Chemotherapy and radiotherapy protocols continue to play a primary role in the current conventional treatment scheme.Hormonal and targeted therapies have also been introduced into the treatment plan in recent years (3, 4).With those developments, pathologists have started to play a greater role in the detection of those markers that guide the treatment of breast cancer.Intraoperative frozen section procedure may be required in breast cancer for reasons such as histological diagnosis, the presence of surgical margin positivity and lymph nodes metastasis.Morphological changes related to freezing can be ob-served in tissues undergoing routine processing after a frozen section procedure.However, the effect of the frozen section procedure on the immunoprofile of cells is still a subject of interest.This issue becomes more important in breast carcinomas because immunohistochemical (IHC) analysis guides the treatment.We investigated the effect of frozen section procedure on the oestrogen (ER), progesterone (PR) and HER2 expression in breast carcinoma cells using immunohistochemical methods. MATERIAL AND METHODS Study population, and frozen and routine tissueprocessing A total of 53 cases evaluated with intraoperative frozen sections in our hospital between October 2009 and July 2010 were Background: Intraoperative frozen section procedure may be required in some operations performed for breast masses.Aims: We investigated the effect of frozen section procedure on the immunoprofile of breast carcinoma cells. Study Design: Cross-sectional study.Methods: A total of 53 breast carcinoma cases evaluated with intraoperative frozen sections were included in this study.Immunohistochemically, oestrogen (ER), progesterone (PR) and HER2 primary antibodies were evaluated in both frozen and non-frozen sections of each tumour sample.Results: No difference was found between the frozen and non-frozen sections in 33 cases in terms of staining rate and intensity of ER, PR and HER2.A decrease was found in the ER, PR and HER2 stain-ing rate (in 10 cases, 9 cases and 6 cases, respectively).Likewise, a decrease was detected in ER, PR and HER2 staining intensity in 6 patients for each.Although there was no staining in frozen sections, immunopositivity was observed in 3 non-frozen sections for ER, in 2 for PR and in 5 for HER2.Statistically, a significant difference was found between the frozen and non-frozen sections in terms of staining rate and intensity for each of the three markers.Conclusion: In this study, the frozen section procedure in breast carcinoma had a negative effect on the immunoprofile.However, considering the importance of hormone receptor status in the treatment, these results should be supported with larger series.