Antileishmanial Activity of Selected Turkish Medicinal Plants
Makaleler > Tam Makale · 2014
Yazarlar: Ahmet Ozbilgin, Cenk Durmuskahya, Hüsniye KAYALAR, Hatice Ertabaklar, Cumhur GÜNDÜZ, Ipek Ostan Ural, Fadile Zeyrek, Ozgur Kurt, Ibrahim Cavus, Cuneyt Balcioglu, Seray Ozensoy Toz, Yusuf ÖZBEL
TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH, SCI İndekslerine Giren Dergi, Uluslararası, Wos, 2014, cilt 13, ss 2047-2055
Özet
Purpose: To determine the in vitro and in vivo anti-leishmanial activities of extracts obtained from Centaurea calolepis, Phlomis lycia, Eryngium thorifolium, Origanum sipyleum and Galium incanum ssp. centrale.Methods: To estimate the cytotoxicity of plant extracts, WST-1 assay was used. Parasite inhibition in the presence of plant extracts (25 - 500 µg/ml) in comparision with control group and reference group (glucantime, 25 µg/ml) at 12 - 72 h were determined in vitro on L. tropica promastigotes. The in vivo leishmanicidal activity of the extracts was evaluated against L. tropica-infected mice with glucantime as reference drug.Results: The chloroform extract of Galium incanum ssp. centrale showed the highest cytotoxicity with IC50 value of 0.0316 ± 0.005 µg/ml. In vitro parasite inhibition by the plant extracts ranged between 16.7 ± 0.01 % and 100 ± 0.00 % at 25 µg/ml concentration. The methanol extract of Eryngium thorifolium possessed the highest activity on promastigotes of L. tropica with 100 % inhibition at 25 µg/ml. The water and chloroform extracts of C. calolepis and water and methanol extracts of E. thorifolium at a dose of 100 mg/kg reduced parasitaemia in L. tropica infected mice.Conclusion: Parasite viability results suggest that the methanol extract of Eryngium thorifolium, regarded as non-cytotoxic, is a promising candidate drug for treating L. tropica infection.Keywords: Leishmaniasis, Leishmania tropica, Eryngium thorifolium, Promastigote, WI-38 Human fibroblasts