Electrochemical deposition of polypeptides: bio-based covering materials for surface design
Makaleler > Tam Makale · 2014
Yazarlar: Huseyin Akbulut, Murat YAVUZ, Emine GÜLER ÇELİK, Dilek ODACI, Takeshi ENDO, Shuhei YAMADA, Suna TİMUR, Yusuf Yağcı
POLYMER CHEMISTRY, SCI Expanded İndekslerine Giren Dergi, Uluslararası, 2014, cilt 5, ss 3929-3936
DOI: 10.1039/c4py00079j
Özet
A simple and efficient approach for the electrochemical deposition of polypeptides as bio-based covering materials for surface design is described. The method involves N-carboxyanhydride (NCA) ring-opening polymerization from its precursor to form a thiophene-functionalized polypeptide macromonomer (T-Pala), followed by electropolymerization. The obtained conducting polymer, namely polythiophene-g- polyalanine (PT-Pala), was characterized and utilized as a matrix for biomolecule attachment. The biosensing applicability of PT-Pala was also investigated by using glucose oxidase (GOx) as a model enzyme to detect glucose. The designed biosensor showed a very good linearity for 0.01-1.0 mM glucose. Finally, the antimicrobial activities of newly synthesized T-Pala and PT-Pala were also evaluated by using the disc diffusion method.