Molecularly Imprinted Polypyrrole for DNA Determination
Makaleler > Tam Makale · 2013
Yazarlar: Vilma Ratautaite, Seda Nur Topkaya, Lina Mikoliunaite, Mehmet Ozsoz, Yasemin Oztekin, Almira Ramanaviciene, Arunas Ramanavicius
Electroanalysis, Uluslararası, Wos, 2013, cilt 25, ss 1169-1177
Özet
Abstract In this study graphite electrodes modified by a thin DNA‐imprinted polypyrrole layer, which was able to bind specific target‐DNA, are reported. For this aim, electrochemical synthesis of polypyrrole was performed on a pencil graphite electrode by cyclic voltammetry (CV) or by potential pulse sequences (PPS). The modified electrode surface was used for electrochemical determination of target‐DNA by differential pulse voltammetry. According to our best knowledge this is a first report on the application of DNA‐imprinted polymer for the determination of target‐DNA. The results showed that the molecularly imprinted polypyrrole (MIPPy) layer that formed on the carbon electrode surface was sensitive for target‐DNA, while the nonimprinted polypyrrole layer was not sensitive to the same target‐DNA. Comparison of electrodes modified using PPS and CV techniques is presented.