Immobilization of anti-aflatoxin B1 antibody by UV polymerization of aniline and aflatoxin B1 detection via electrochemical impedance spectroscopy
Makaleler > Tam Makale · 2012
Yazarlar: Erhan DİNÇKAYA, Özer KINIK, Mustafa Kemal Sezginturk, Çağrı ALTUĞ, Aylin Akkoca
Artificial Cells, Blood Substitutes, and Biotechnology, SCI Expanded İndekslerine Giren Dergi, Uluslararası, Wos, 2012, cilt 40, ss 385-390
DOI: 10.3109/10731199.2012.696059
Özet
In the study, we investigated the practicality of the UV polymerization of aniline for anti-aflatoxin B1 antibody immobilization, and utilization of the resulting biosensor in the impedimetric determination of aflatoxin B1. The anti-aflatoxin B 1 antibody was physically immobilized on gold electrodes by UV polymerization of aniline at a fixed wavelength. The biosensor was based on specific interaction anti-aflatoxin B1 - aflatoxin B1 recognition and investigation of this recognition event by electrochemical impedance spectroscopy. A calibration curve was obtained in a linear detection range 1-20 ng/mL aflatoxin B1. Finally, the biosensor was applied to analysis of a real food sample.