Investigation of Antimicrobial Effects of Organic Antibiotic
Bildiriler > Özet Bildiri · 2022
Yazarlar: Kadriye Buşra Karatay, Nihal Doğruöz Güngör, Batu Çolak, Serhan Sakarya, Fazilet Zümrüt BİBER MÜFTÜLER
4th Eurasia Biochemical Approaches & Technologies (EBAT), Türkiye, Antalya, Uluslararası, Sözlü Sunum, Özet Bildiri, 2022-2022, ss 70-70
Özet
<p>Some radionuclides used in nuclear medicine can be combined (radiolabeled) with molecules that are </p><p>selective by cancerous or infected tissues, for the development of targeted drugs, as well as image </p><p>functionality, and can be directed to these tissues.</p><p>Antibiotics have been used for many years in the treatment of infectious diseases. However, as a result </p><p>of the widespread use of wrong and unnecessary antibiotics in the world and in our country, there is </p><p>a rapid increase in antibiotic resistance. For this reason, innovative approaches are needed especially </p><p>against microorganisms with biofilm structure and in the diagnosis and treatment of infectious </p><p>diseases. One of these innovative approaches is "alternative to today's synthetic antibiotics, isolation </p><p>of organic antibiotics from cave bacteria and development of innovative agents for imaging the </p><p>infected area".</p><p>The antimicrobial activity of isolated organic cipro (o-CIP) produced by Micrococcus luteus bacteria </p><p>collected from the “Yarık Dudeni Cave” in Mersin, Turkey. After produced organic antibiotics by </p><p>isolated bacterial species were determinated, the structural analysis was done by Liquid </p><p>Chromatography Quadrupole Time-Of-Flight Mass Spectrometry (LC/QTOF/MS) and High Performance </p><p>Liquid Chromatography (HPLC) Analysis. According to LC/QTOF/MS, there was three organic antibiotics </p><p>(Levofloxacin, Clindamycin and Ciprofloxacin). </p><p>Within the scope of the study, the isolation of organic antibiotics produced by Micrococcus luteus from </p><p>bacterial media, the isolated organic antibiotics against pathogenic microorganisms (Escherichia coli </p><p>(ATCC 9637), Staphylococcus aureus (ATCC 29213), Pseudomonas aeruginosa (ATCC 39327), </p><p>Vancomycin Resistant Enterococci (ATCC MP-1) , SCCmec Type MRSA (ATCC MP-2), Candida albicans </p><p>(ATCC 10231)), radiolabeling of isolated organic antibiotics, antimicrobial activity of radiolabeled </p><p>antibiotics on these pathogenic microorganisms were investigated. </p><p>Organic antibiotic were radiolabelled demonstrated higher yield than synthetic derivative. Quality </p><p>control studies were examined using the TLRC method and it was determined that both substances </p><p>remained stable for 240 minutes. The % binding efficiency of radiolabeled antibiotics on pathogenic </p><p>microorganisms were determined and compared with each other. </p><p>This study supported by “Ege University, Office of Scientific Research Projects (BAP)”, Project No: FMDKT-2022-23843.</p>