Synthesis, characterization and biodistribution of Tc-99m-Bioquin-HMPAO (Tc-99m-BH) as a novel brain imaging agent
Makaleler > Tam Makale · 2014
Yazarlar: Ayfer YURT KILÇAR, Fazilet Zumrut Biber MUFTULER, Huseyin Enginar, Volkan TEKIN, Emin İlker MEDİNE, Perihan ÜNAK
JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY, SCI Expanded İndekslerine Giren Dergi, Uluslararası, 2014, cilt 302, ss 563-573
DOI: 10.1007/s10967-014-3175-1
Özet
Recently, the development of novel brain imaging agents has aroused much interest thanks to limited number of brain cancer or diseases diagnosis agents. It is aimed to synthesize a novel brain imaging agent including a promise for further studies on AD diagnosis potential and investigate its bioaffinity with biodistribution studies on healthy Balb/c mice. A novel radiolabeled agent was synthesized and characterized. Quality control of Tc-99m-BH was performed utilizing solvent extraction and chromatographic (Radio TLC and Radio HPLC) methods. Bioaffinity of the Tc-99m-BH was investigated on male Balb/c mice at various time points (5, 30, 60, 120 min post-injection). Paper electrophoresis showed that Tc-99m-BH has a neutral structure. Radiochemical purity of Tc-99m-BH was over 95 % with appropriate stability for imaging period. Selected brain regions have uptakes over 4 % ID/g following intravenous injection. Hippocampus has uptake approximately 10 % ID/g. Tc-99m-BH has shown brain uptake, so it may prove to be valuable for brain imaging as a novel technetium-labeled agent. Further investigations with AD animal model are our on going effort to show that this agent has AD diagnosis potential.