Affinity Based Laccase Immobilization on Modified Magnetic Nanoparticles: Biosensing Platform for the Monitoring of Phenolic Compounds
Articles > Article · 2014
Authors: Alper BABADOSTU, Özge KOZGUŞ GÜLDÜ, Dilek ODACI, Emin İlker MEDİNE, Perihan ÜNAK, Suna TİMUR
INTERNATIONAL JOURNAL OF POLYMERIC MATERIALS AND POLYMERIC BIOMATERIALS, Journal Indexed in SCI Expanded, International, WoS, 2014, vol 64, pp 260-266
DOI: 10.1080/00914037.2014.936598
Summary
The authors report an electrochemical phenol biosensor based on the immobilization of laccase (Lac) on the surface of copper capped magnetic core-shell (Fe3O4-SiO2) nanoparticles (MNPs). After synthesis, MNP surfaces were functionalized by silanization and then, modified with histidine (His) and copper, respectively. The performance of the MNP-His/Cu/Lac biosensor has been investigated at -0.05V versus Ag/AgCl. The proposed biosensor allowed to detect catechol and phenol in the range of 0.01-0.4mM and 0.025-0.2mM, respectively. Finally, phenol analysis in culture medium of P. putida adapted to phenol was successfully demonstrated with appreciable recovery values.