Isolation and Immobilization of His-Tagged Alcohol Dehydrogenase on Magnetic Nanoparticles in One Step: Application as Biosensor Platform
Articles > Article · 2014
Authors: Özge KOZGUŞ GÜLDÜ, Selin ECE, Serap EVRAN, Emin İlker MEDİNE, Dilek ODACI, Perihan ÜNAK, Suna TİMUR
JOURNAL OF MACROMOLECULAR SCIENCE PART A-PURE AND APPLIED CHEMISTRY, Journal Indexed in SCI Expanded, International, WoS, 2014, vol 51, pp 699-705
DOI: 10.1080/10601325.2014.936236
Summary
His-tagged Alcohol dehydrogenase was produced as a recombinant protein in E. coli. Afterwards, isolation and immobilization of the enzyme was carried in one-step via copper modified magnetic nanoparticles (MNPs) by the effect of interactions between Cu and histidine. The resulting enzyme bound MNPs was then attached to the surface of carbon paste electrode by the magnetic force and used as an electrochemical biosensor for the alcohol sensing applications.