Bioassay-guided isolation and identification of Aedes aegypti larvicidal and biting deterrent compounds from Veratrum lobelianum
Makaleler > Tam Makale · 2018
Yazarlar: Nurhayat Tabanca, Zulfiqar Ali, Ulrich R. Bernier, Nancy Epsky, Ayse Nalbantsoy, Ikhlas A. Khan, Abbas Ali
Open Chemistry, Uluslararası, Wos, 2018, cilt 16, ss 324-332
Özet
Abstract An ethanol extract from Veratrum lobelianum Bernh. rhizomes showed larvicidal activity with LC 50 values of 11.79 ppm and 89.9 ppm against 1 st and 4 th instar larvae, respectively, at 24 h post-treatment. The extract also showed proportion not biting (PNB) value of 0.76 at 100 μg/cm 2 against females of Ae . aegypti . Systematic bioassay-guided fractionation of V . lobelianum extract resulted in the isolation of five compounds that were identified as ethyl palmitate ( 1 ), ethyl linoleate ( 2 ), β-sitosterol ( 3 ), resveratrol ( 4 ) and oxyresveratrol ( 5 ) by GC-MS, 1 H-NMR, and 13 C-NMR techniques, comparison with literature data, and confirmation with authentic compounds. Compound 2 exhibited larvicidal activity with an LC 50 value of 24.1 (22.0-26.2) ppm whereas 1 was inactive. β-Sitosterol ( 3 ) displayed the highest larvicidal activity with LC 50 = 1.7 (1.3-12.3) ppm and LC 90 = 5.1 (3.4-13.8) ppm. Compounds 4 and 5 had larvicidal activity with LC 50 values of 18.5 (15.3-23.3) and 22.6 (19.0-26.8) ppm, respectively, and had an equivalent PNB values of 0.75 at 25 nmol/cm 2 . In addition, 4 and 5 were explored for their human-based repellency against Ae . aegypti , attractiveness against male medflies Ceratitis capitata , and also evaluated against series of human carcinoma cells (A549, HEK293, HeLa, SH-SY5Y); however, no significant activity was found.