Accumulation of α-Synuclein in Cerebellar Purkinje Cells of Diabetic Rats and Its Potential Relationship with Inflammation and Oxidative Stress Markers
Makaleler > Tam Makale · 2017
Yazarlar: Volkan Solmaz, Hatice Kose Ozlece, Huseyin Avni Eroglu, Hüseyin AKTUĞ, Oytun Erbas, Dilek TAŞKIRAN
Neurology Research International, SCI Expanded İndekslerine Giren Dergi, Uluslararası, Scopus, 2017, cilt 2017, ss 1-6
DOI: 10.1155/2017/5952149
Özet
Objective. The present study was conducted to evaluate the relationship between plasma oxidative stress markers such as malondialdehyde (MDA) and glutathione (GSH), inflammatory marker pentraxin-3 (PTX3), and cerebellar accumulation of α-synuclein in streptozotocin- (STZ-) induced diabetes model in rats. Methods. Twelve rats were included in the study. Diabetes ( n=6 ) was induced with a single intraperitoneal injection of streptozotocin (STZ, 60 mg/kg). Diabetes was verified after 48 h by measuring blood glucose levels. Six rats served as controls. Following 8 weeks, rats were sacrificed for biochemical and immunohistochemical evaluation. Results. Plasma MDA levels were significantly higher in diabetic rats when compared with the control rats ( p<0.01 ), while plasma GSH levels were lower in the diabetic group than in the control group ( p<0.01 ). Also, plasma pentraxin-3 levels were statistically higher in diabetic rats than in the control rats ( p<0.01 ). The analysis of cerebellar α-synuclein immunohistochemistry showed a significant increase in α-synuclein immunoexpression in the diabetic group compared to the control group ( p<0.01 ). Conclusion. Due to increased inflammation and oxidative stress in the chronic period of hyperglycemia linked to diabetes, there may be α-synuclein accumulation in the cerebellum and the plasma PTX3 levels may be assessed as an important biomarker of this situation.