Determination of the Genetic Diversity of the Lactoferrin Gene Polymorphism in Indigenous Anatolian Goat Breeds
Makaleler > Tam Makale · 2012
Yazarlar: Iraz Akış Akad, Feraye Esen Gürsel, Cemal ÜN, Kemal Özdem Öztabak
Thai Journal of Agricultural Science, AHCI İndekslerine Giren Dergi, Uluslararası, 2012, cilt 45, ss 83-88
Özet
<p>Lactoferrin (LF) gene is considered as a candidate gene for mastitis resistance due to the role of LF in defence mechanism of the mammary gland. LF gene is highly conserved among mammals. Studies have focused to analyze the diversity of LF gene polymorphisms in different breeds. There are limited data about genetic diversity, phylogeny and candidate genes in Anatolian goat breeds. In this study four indigenous Anatolian goat breeds were genotyped for 7605 C>T polymorphism in exon 4 of LF gene using PCR-RFLP method and direct sequencing. Two alleles C and T were observed, which are resulting Arginine and Tryptophan, respectively. T allele frequency was found to be higher in all the breeds, varied between 81.25 and 93.75%. CC genotype was the least observed genotype and it was absent in Hair goat breed. The populations except Hair goat were found to be in Hardy-Weinberg equilibrium. The genetic diversity of the analyzed site was found to be high except Kilis breed. Investigation of the genetic diversity of Anatolian goat breeds with different parameters would contribute the genetic database of goat breeds. Further studies on caprine LF gene and its associations with mastitis in Anatolian goat breeds should be conducted. <br></p>