INVESTIGATION OF IN VITRO PROPAGATION OF CAMPANULA LEBLEBICI VIA PLANTFORM TEMPORARY IMMERSION SYSTEM

Bildiriler > Özet Bildiri · 2023

Yazarlar: Funda Koçer, Başar Sevindik, Özhan Şimşek, Ademi Fahri PİRHAN

100th ANNIVERSARY OF THE REPUBLIC TURKEY INTERNATIONAL EGE AGRICULTURE CONGRESS, Türkiye, İzmir, Uluslararası, Sözlü Sunum, Özet Bildiri, 2023-2026, ss 34-35

DOI: 10.5281/zenodo.10397164

Özet

<p>The&nbsp;family&nbsp;Campanulaceae&nbsp;exhibits&nbsp;a&nbsp;cosmopolitan&nbsp;distribution&nbsp;from&nbsp;tropical&nbsp;to&nbsp;cold&nbsp;regions&nbsp;across&nbsp;all&nbsp;continents.&nbsp;Campanulaceae&nbsp;is&nbsp;divided&nbsp;into&nbsp;three&nbsp;subfamilies&nbsp;Campanula&nbsp;leblebicii,&nbsp;discovered&nbsp;by&nbsp;Yıldırım,&nbsp;is&nbsp;found&nbsp;only&nbsp;in&nbsp;the&nbsp;village&nbsp;of&nbsp;Ovacık&nbsp;in&nbsp;the&nbsp;Kemalpaşa&nbsp;district&nbsp;of&nbsp;İzmir&nbsp;province&nbsp;and&nbsp;it&nbsp;is&nbsp;one&nbsp;of&nbsp;the&nbsp;valuable&nbsp;endemic&nbsp;species&nbsp;for&nbsp;Turkey&nbsp;flora.&nbsp;Biotechnological&nbsp;methods&nbsp;are&nbsp;very&nbsp;effective&nbsp;for&nbsp;preserving&nbsp;and&nbsp;sustainability&nbsp;of&nbsp;genetic&nbsp;resources.&nbsp;Tissue&nbsp;culture&nbsp;is&nbsp;one&nbsp;of&nbsp;the&nbsp;important&nbsp;methods&nbsp;for&nbsp;this&nbsp;purpose.&nbsp;Petiole&nbsp;and&nbsp;leaf&nbsp;explants&nbsp;were&nbsp;cultured&nbsp;on&nbsp;full&nbsp;strength&nbsp;MS&nbsp;medium&nbsp;containing&nbsp;30&nbsp;gL-1&nbsp;sucrose,&nbsp;4&nbsp;gL-1&nbsp;gelrite,&nbsp;different&nbsp;concentrations&nbsp;of&nbsp;6-benzyladenine&nbsp;(BA)&nbsp;(0.&nbsp;0.5,&nbsp;1,&nbsp;2,&nbsp;3&nbsp;mgL-1&nbsp;)&nbsp;for&nbsp;callus&nbsp;initiation.&nbsp;Different&nbsp;immersion&nbsp;(10,&nbsp;15,&nbsp;20&nbsp;min)&nbsp;and&nbsp;ventilation&nbsp;(5,&nbsp;10,&nbsp;15&nbsp;min)&nbsp;time&nbsp;was&nbsp;investigated&nbsp;on&nbsp;efficient&nbsp;regeneration&nbsp;of&nbsp;C.&nbsp;leblebici.&nbsp;Efficient&nbsp;callus&nbsp;and&nbsp;shoot&nbsp;proliferation&nbsp;were&nbsp;observed&nbsp;on&nbsp;MS&nbsp;medium&nbsp;including&nbsp;3&nbsp;mgL-1&nbsp;BA&nbsp;+0.1&nbsp;mgL-1&nbsp;NAA&nbsp;as&nbsp;100%.&nbsp;Callus&nbsp;were&nbsp;transferred&nbsp;to&nbsp;the&nbsp;Plantform&nbsp;Temporary&nbsp;Immersion&nbsp;bioreactor&nbsp;including&nbsp;3&nbsp;mgL-1&nbsp;BA&nbsp;+0.1&nbsp;mgL-1&nbsp;NAA&nbsp;after&nbsp;solid&nbsp;medium.&nbsp;Adventive&nbsp;root&nbsp;formations&nbsp;were&nbsp;observed&nbsp;spontaneously&nbsp;on&nbsp;MS&nbsp;medium&nbsp;3&nbsp;mgL-1&nbsp;BA&nbsp;+0.1&nbsp;mgL-1&nbsp;NAA.&nbsp;Callus&nbsp;volume&nbsp;increased,&nbsp;shoot&nbsp;formations&nbsp;and&nbsp;root&nbsp;formations&nbsp;increased&nbsp;in&nbsp;temporary&nbsp;immersion&nbsp;system.&nbsp;Efficient&nbsp;callus,&nbsp;shoot&nbsp;and&nbsp;roots&nbsp;were&nbsp;observed&nbsp;on&nbsp;15&nbsp;min&nbsp;immersion&nbsp;and&nbsp;10&nbsp;min&nbsp;ventilation&nbsp;time.&nbsp;Keywords:&nbsp;Campanula,&nbsp;MS,&nbsp;Plantform,&nbsp;Temporary&nbsp;immersion&nbsp;system,&nbsp;NAA&nbsp;</p>

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