Classical and Molecular Diagnosis of Pseudomonas syringae Pathovars Causing Bacterıal Canker on Stone Fruits
Makaleler > Tam Makale · 2020
Yazarlar: Ertimurtaş, Damla, Hatice ÖZAKTAN
DergiPark (Istanbul University), Uluslararası, 2020
Özet
15 Pseudomonas syringae (Ps) pathovars isolated from stone fruit trees such as peach, almond and cherry with bacterial cancer symptoms and kept in the Bacteriology laboratory stocks of Ege University Faculty of Agriculture, Department of Plant Protection were identified based on LOPAT (levan, oxidase, potato rot pectolytic activity, arginin dihydrolase, hypersensitive reaction on tobacco leaves), GATTa (gelatin liquefaction, aesculin hydrolysis, tyrosinase activity, utilization of tartaric acid), utilization of carbon tests classical methods.In addition, pathogenicity, syringomycin production and ice nucleation activity (INA) were used for the identification.Pseudomonas syringae pathovars were identified by PCR that cannot be diagnosed with classical tests.Molecular diagnosis of Pseudomonas syringae pv.syringae (Pss) strains were based on syringomycin synthesis (syrB) and Pseudomonas syringae pv.morsprunorum (Psm) strains were based on coronatine synthesis (cfl) were diagnosed using PCR.Also sequence analysis of 4 housekeeping genes (cts, rpoD, gapA, gyrB) were used to distinguish Pss and Psm.As a result of classical and molecular identification 9 of 15 Ps pathovars were identified as Pss.