Buttermilk based cobalt phthalocyanine dispersed ferricyanide mediated amperometric biosensor for the determination of xanthine
Makaleler > Tam Makale · 1998
Yazarlar: Mustafa Emrah KILINÇ, Kadriye Arzum ERDEM GÜRSAN, L Gokgunnec, T Dalbasti, M Karaoglan, M Ozsoz
ELECTROANALYSIS, SCI Expanded İndekslerine Giren Dergi, Uluslararası, Wos, 1998, cilt 10, ss 273-275
DOI: 10.1002/(SICI)1521-4109(199804)10:4<273::AID-ELAN273>3.0.CO;2-O
Özet
A tissue-based amperometric biosensor for xanthine determination has been developed. The biosensor uses a carbon paste electrode (CPE) which contains buttermilk (BTM) as source of xanthine oxidase (XOD) (EC.1.1.3.2.2.) and cobalt phthalocyanine (CoPc) as mediator. The system is also mediated with ferricyanide in solution to obtain a double-mediated biosensor. The effect of various variables upon the response was studied. Linearity was observed over the concentration range of 1-15 mM xanthine. The optimum operational pH range for the electrode is 8.0-9.0. Reproducibility was studied and a relative standard deviation (rsd) of 10.26 % was found. After 60 days the normalized response of the BTM-modified electrode was 60 % of the first day.