Optimization of Plant Regeneration in Different Pepper (Capsicum annuum L.) Lines
Makaleler > Tam Makale · 2020
Yazarlar: Tolga İzgü, Hülya İLBİ, Yeşim Yalçın Mendi
Turkish Journal of Agriculture - Food Science and Technology, Uluslararası, 2020, cilt 8, ss 471-477
DOI: 10.24925/turjaf.v8i2.471-477.3207
Özet
Development of an efficient plant regeneration protocol is essential for vegetable crop advancement by biotechnological methods. In this study, regeneration protocols of four pepper lines of different pepper types were optimized. Different protocols for organogenesis were investigated in regeneration experiments. Optimum plant regeneration was obtained in different combinations of 6-Benzylaminopurine (BAP) and Indole-3-acetic acid (IAA) in organogenesis assays. In organogenesis experiment, the highest shoot development was determined as 80% from hypocotyl explant of Demre pepper in 4 mg L-1 BA+0.5 mg L-1 IAA, 80% from hypocotyl explant of Charleston pepper in 3 mg L-1 BA+0.5 mg L-1 IAA, 80% from hypocotyl explant of capia pepper in 5 mg L-1 BA+0.5 mg L-1 and 84% from hypocotyl explant of bell pepper in 5 mg L-1 BA+0.5 mg L-1 IAA. Afterward, shoots were rooted and whole plants were obtained.