EFFECTS OF NICKEL CHLORIDE ON CELL MORPHOLOGY AND MIGRATION IN NON-SMALL CELL LUNG CANCER CELL LINES
Makaleler > Tam Makale · 2023
Yazarlar: Hakan Turan Kiriş, Çağlanur Taşkaya, Adil Bahadır, Erdem GÖKER
TURKISH MEDICAL STUDENT JOURNAL, Uluslararası, 2023, cilt 10, ss 112-115
DOI: 10.4274/tmsj.galenos.2023.2022-12-3
Özet
Aims: The discovery of the anti-cancer properties of the metal-based compound cisplatin and its effective use in cancer treatment has opened the door to the investigation of the anti-cancer properties of many other metals and metal-based compounds.Studies have shown that nickel chloride (NiCl 2 ) could suppress cell migration and metastasis in some types of cancer and could even be a promising anti-cancer agent in oral cancers, although the activity of NiCl 2 on the cell morphology and cell migration in non-small cell lung cancer cell lines (A549) is unknown.Thus, we aimed to investigate the role of NiCl 2 on cell morphology and migration in non-small cell lung cancer cell lines.Methods: The present study investigates the effect of NiCl 2 on cell morphology and cell migration in A549 cell lines using a Giemsa staining technique, with an in vitro scratch analysis performed to determine the effect of NiCl 2 on cell migration.Results: No significant change was observed in cell morphology in the group treated with 200 µM NiCl 2 compared to the control group, while the cellular morphology was changed in the cell lines treated with 600 µM NiCl 2 .The cells lost cell-to-cell contacts, the cytoplasm shrank, and their morphology diverged from that of their ancestors, taking on a spindle-shaped and more unhealthy appearance.In addition, it was observed that cell confluency was decreased by half.It was found that NiCl 2 treatment at a dose at which cell morphology changed (600 µM) significantly reduced cell migration after 12 hours, and the effect was sustained at 24 and 48 hours, with cell migration significantly suppressed.Conclusion: Our results suggested that treatment of non-small cell lung cancer cell lines with NiCl 2 changed cell morphology in a dose-dependent manner and suppressed the migration of cancer cells.