Kalsiyum hidroksit-sığır dentin tanesinin hücre canlılığı, inflamatuar tepkisi ve antimikrobiyal aktivitesinin karşılaştırmalı değerlendirilmesi.
Makaleler > Tam Makale · 2024
Yazarlar: Mustafa ATEŞ, Çiğdem ATALAYIN ÖZKAYA, Güliz ARMAGAN, Dilek Akin, Derviş BİRİM, Taner Dagci, Hüseyin TEZEL
TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI, SCI Expanded İndekslerine Giren Dergi, Uluslararası, Scopus, 2024, cilt 49, ss 647-655
DOI: https://doi.org/10.1515/tjb-2024-0040
Özet
<p>Objectives: Dentin tissue can act as a reservoir for bioactive</p><p>molecules that create signals for cellular proliferation and</p><p>differentiation to initiate tissue regeneration. Therefore, the</p><p>aim of this study is to compare the cell viability, inflammatory</p><p>response, and antimicrobial activity of bovine dentin</p><p>grain-added calcium-hydroxide (CH-BDG) with different</p><p>pulp-capping materials.</p><p>Methods: ProRoot MTA, Biodentine, Dycal, TheraCal-LC,</p><p>and an experimental material, CH-BDG, were examined. Cell</p><p>viability was determined via the WST-1 assay. The inflammatory</p><p>response was analysed by the monocyte chemoattractant</p><p>protein-1 (MCP-1/CCL2) andmacrophage inflammatory protein-</p><p>1α (MIP-1α/CCL3) levels. The antimicrobial activity was</p><p>evaluated by agar-diffusion.</p><p>Results: The cell viability of CH-BDG was analogous with</p><p>Biodentine and control at 24 h. The cell viability of CH-BDG</p><p>decreased at 48 h, but the rate was higher than ProRoot MTA</p><p>and Dycal (p<0.05). For MCP-1 and MIP-1α values, there was</p><p>no significant difference between the control and CH-BDG.</p><p>The MCP-1 level of CH-BDG was lower compared to other</p><p>pulp-capping materials (p<0.05). The MIP-1α level of CH-BDG</p><p>was lower compared to ProRoot MTA, Biodentine, and</p><p>TheraCal-LC (p<0.05). No inhibition zone was detected</p><p>against oral microorganisms for CH-BDG.</p><p>Conclusions: The experimentally developed CH-BDG showed</p><p>competing properties and additional advantages compared to</p><p>the existing pulp-capping materials.</p><p>Keywords: biomaterial; biocompatibility; chemokine level;</p><p>experimental material; pulp capping</p>