Investigation of Propagation Possibilities of Paulownia tomentosa in Plantform and SETIS Bioreactor Systems
Bildiriler > Özet Bildiri · 2025
Yazarlar: Ramazan ŞEN, Başar Sevindik, Hasan YILDIRIM, Ademi Fahri PİRHAN
IConPB 2025 4 th International Congress on Plant Biology, Türkiye, Denizli, Uluslararası, Sözlü Sunum, Özet Bildiri, 2025-2025, ss 27-28
Özet
<p>Abstract This study was conducted to determine the optimum immersion and aeration frequency using SETIS and Plantform bioreactors for large-scale, rapid and clonal propagation of commercially important Paulownia tomentosa. The use of bioreactors is important to overcome the problems that arise from traditional propagation methods and micropropagation studies using small vessels or tubes. In the study, 40 nodal segment explants were tested in SETIS and Plantform bioreactors, 3 different immersion and aeration frequencies (10-minute immersion every 3/4/6 hours, 5-minute aeration (Plantform)) and observations were taken at the end of 4 weeks, according to the randomized plots experimental design with 3 replications. MS nutrient medium containing 1mg/L BAP, pH 5.8 was used. All cultures were maintained at 25 ±2°C, 16/8h photoperiod. The observation parameters in the study are shoot regeneration, shoot number and length, leaf number and length, fresh mass. The best result was obtained from Plantform bioreactor with 10 minutes immersion and 5 minutes aeration applied every 3 hours and it was found that this bioreactor is more effective for large scale production of Paulownia. Keywords: Paulownia sp, bioreactor, Plantform, SETIS, micropropagation </p>