From Invisible Structures of SWCNTs toward Fluorescent and Targeting Architectures for Cell Imaging
Makaleler > Tam Makale · 2013
Yazarlar: Didem Ag, Muharrem Seleci, Rebecca Bongartz, Mustafa Can, Seda Yurteri, Ioan Cianga, Frank Stahl, Suna TİMUR, Thomas Scheper, Yusuf Yağcı
BIOMACROMOLECULES, SCI Expanded İndekslerine Giren Dergi, Uluslararası, 2013, cilt 14, ss 3532-3541
DOI: 10.1021/bm400862m
Özet
Single-walled carbon nanotubes (SWNTs) are unique nanostructures used as cargo systems for variety of diagnostic and therapeutic agents. For taking advantage of these structures in biological processes, they should be visible. Therefore, fluorescence labeling of SWCNTs with various probes is a significant issue. Herein, we demonstrate a simple approach for cell specific imaging and diagnosis by combining SWCNTs with a copolymer poly(para-epsilon-caprolactone) (PCL) side chains (PPP-g-PSt-PCL). In this approach PPP-g-PSt-PCL is noncovalently attached on carboxyl functional SWCNTs. The obtained fluorescent probe is bound to folic acid (FA) for targeted imaging of folate receptor (FR) positive HeLa cells. In vitro studies demonstrate that this conjugate can specifically bind to HeLa cells and indicate great potential for targeting and imaging studies.