Differential Impact of Staphylococcus aureus Infection on Lung Cancer and Normal Bronchial Cells
Bildiriler > Özet Bildiri · 2025
Yazarlar: Deniz Ece, Günel Mukhtarova, Ufuk MERT, Gökhan Gurur Gökmen, Tuncay GÖKSEL, Ayşe CANER
Annual Congress of the European Association for Cancer Research, Portekiz, Lizbon, Uluslararası, Poster, Özet Bildiri, 2025-2025, ss ---
Özet
<p><strong>Introduction:</strong><em> Staphylococcus aureus(S. aureus)</em>infection is a frequent complication in lung cancer patients, accounting for up to 70% of infectious issues. Traditionally seen as an extracellular pathogen, <em>S. aureus</em> is now recognized for its ability to survive and replicate intracellularly within tumor tissues. This intratumor invasion can trigger inflammatory pneumonia, obstructive pulmonary diseases, and secondary infections, potentially leading to organ dysfunction. Despite advances in cancer treatments, these infections remain a major cause of mortality, emphasizing the urgent need for improved strategies to manage bacterial complications in lung cancer patients. This study aims to elucidate the molecular and cellular effects of <em>S. aureus</em> infection on lung cancer and normal bronchial epithelial cells.</p><p><br></p><p><strong>Material and Methods</strong>: The effects of <em>S. aureus</em> infection at 1:25, 1:50, and 1:100 ratios on A549 lung cancer and BEAS-2B bronchial epithelial cell lines were established. Intracellular infection was visualized using Giemsa staining. The intracellular <em>S. aureus</em> burden was quantified through CFU analysis, and the infection index was subsequently calculated. Cell cycle and apoptosis assay using flow cytometry. Immunofluorescence staining was conducted to detect DNA damage induced by the infection. To assess metastatic potential, colony formation assays, invasion and migration assays were performed. CD44/133 staining was utilized to explore stem cell-like properties. The expression levels of key genes involved in cancer progression, apoptosis, and migration were examined using RT-PCR.</p><p><br></p><p><strong>Results</strong>: The findings of this study highlight the significant effects of <em>S. aureus</em> infection at varying rates on both the A549 and the BEAS-2B cell lines. The results demonstrated that <em>S. aureus</em> infection significantly impacted various cellular processes, including apoptosis, cell cycle, DNA damage, and metastatic / stemness potential in A549 cells than BEAS-2B cells. Additionally, alterations in gene expression related to cancer progression, apoptosis, and migration were detected, providing valuable insights into the molecular mechanisms underlying the interaction between <em>S. aureus</em> and both lung cancer and normal bronchial epithelial cells. These findings contribute to a deeper understanding of the role of bacterial infection in cancer biology and its potential implications for cancer progression and treatment.</p><p><br></p><p><strong>Conclusion: </strong>This study revealed that <em>S. aureus</em> infects both lung cancer and normal bronchial epithelial cells, but lung cancer cells are more vulnerable to intracellular invasion and dysfunction. These differential effects underscore the need for further research into the underlying molecular mechanisms to develop targeted therapies that address both cancer progression and associated bacterial infections, ultimately improving outcomes for lung cancer patients.</p>