Usage of Bismuth Film Electrode as Biosensor Transducer for Alkaline Phosphatase Assay
Makaleler > Tam Makale · 2010
Yazarlar: Ülkü Anik, Meliha Çubukçu, Serdar Çevik, Suna Timur
Electroanalysis, Uluslararası, Wos, 2010, cilt 22, ss 1519-1523
Özet
Abstract A biosensor for monitoring alkaline phosphatase (ALP) enzyme activity was developed by using bismuth film electrode (BiFE) as transducer. The aim was to test the possible applications of BiFE as transducer in this biosensing platform. ALP was immobilized onto the BiFE electrode by means of gelatin which was then crosslinked by glutaraldehyde. The measurement was based on monitoring of p‐nitrophenol which was released after enzymatic reaction of p‐nitrophenyl phosphate (pNPP) substrate. The effect of enzyme activity (in Unit) was examined for 100 μM of pNPP substrate and a good linearity was observed between 1.28 and 4.26 U activities with the equation of y=0.214x−0.201 and R2=0.998 where x and y show enzyme activity in unit and current response in μA respectively. Analytical characteristics were also examined after the optimization of experimental parameters.