Formation of Corneal Stromal-Like Assemblies Using Human Corneal Fibroblasts and Macromolecular Crowding
Kitapta bölümler > Diğer · 2020
Yazarlar: Gülinnaz ERCAN, Mehmet Gürdal, Dimitrios I Zeugolis
Methods in Molecular Biology, Diğer, Springer Science+Business Media, B, Uluslararası, 2020, ss 119-141, İngiltere, Hertfordshire
DOI: 10.1007/978-1-0716-0599-8_9
Özet
<p>Tissue engineering by self-assembly allows for the formation of living tissue substitutes, using the cells' innate capability to produce and deposit tissue-specific extracellular matrix. However, in order to develop extracellular matrix-rich implantable devices, prolonged culture time is required in traditionally utilized dilute ex vivo microenvironments. Macromolecular crowding, by imitating the in vivo tissue density, dramatically accelerates biological processes, resulting in enhanced and accelerated extracellular matrix deposition. Herein, we describe the ex vivo formation of corneal stromal-like assemblies using human corneal fibroblasts and macromolecular crowding.</p>