Fermentation medium optimization for 1,3-propanediol production using taguchi and box-behnken experimental designs
Makaleler > Tam Makale · 2010
Yazarlar: Mine Gungormusler, Cagdas Gonen, Güven ÖZDEMİR, Nuri AZBAR
FRESENIUS ENVIRONMENTAL BULLETIN, SCI Expanded İndekslerine Giren Dergi, Uluslararası, Scopus, 2010, cilt 19, ss 2840-2847
Özet
Klebsiella species are excellent 1,3-propanediol (1,3 PDO) producers. Klebsiella pneumoniae is a well-studied microorganism and, therefore, its metabolic pathway is known. Accordingly, a number of by-products are simultaneously produced together with 1,3 PDO. Optimization of medium composition significantly decreased these byproducts' concentrations. Optimization of culture media concentrations on 1,3 PDO production by K pneumoniae was studied using statistical experimental designs and analysis, namely, Taguchi Orthogonal Array (TOA) and BoxBehnken design (BBD). The optimal composition of the fermentation medium for maximum 1,3 PDO yield, with a L18 (2∧1 × 3∧7) design using TOA, and after that, a threelevel three-factor BBD, was obtained. The most significant chemicals were found to be glycerol (p<0.0001) and K2HPO4 (p<0.05). MgSO 4.7H2O, which is the third significant factor obtained from TOA, was not found to be significant (p>0.05) from BBD. A maximum 1,3 PDO concentration of 23.6 g/L was obtained which is higher compared to that foreseen by the statistical model (9.3 g/L). © by PSP.