In vitro study of urease/AlaDH enzyme system encapsulated into human erythrocytes and research into its medical applications
Articles > Article · 2002
Authors: Şenay ŞANLIER, AH Uslan
ARTIFICIAL CELLS BLOOD SUBSTITUTES AND IMMOBILIZATION BIOTECHNOLOGY, Journal Indexed in SCI Expanded, International, 2002, vol 30, pp 71-77
Summary
In our system, urease/AlaDH have been encapsulated within erythrocytes by using slow dialysis methods. Urea is decomposed into ammonia and bicarbonate and the ammonia released is converted into alanine by reacting pyruvate under the catalytic action of AlaDH. It is very important for our that products are formed quickly but the ammonia is not connected definetely. For this aim. urease/AlaDH we encapsulated using different enzyme activity ratio (0.5:1.5; 0.5:2.5; 0.25:1.25 U/U urease/AlaDH). The activities of enzyme systems, encapsulation yield, McV, McH, and McHc were measured for each sample. Investigated results suggest that loaded enzyme systems can be used as potential carrier systems for the removal of high levels of urea from blood.