Secondary metabolites from endophytic fungus Penicilium roseopurpureum and investigation of their cytotoxic activities
Makaleler > Tam Makale · 2022
Yazarlar: G Üner, B Dizmen, M Küçüksolak, P B Kırmızıbayrak, E Bedir
Planta Medica, Uluslararası, Wos, 2022, cilt 88, ss 1497-1498
Özet
Endophytes are microorganisms, which colonizes in the internal and distinct tissues of the host plants. They attracted attention as a new source for drug discovery and development due to their rich metabolic profiles consisting of novel and bioactive compounds [1], [2]. In our preliminary study, the chemical diversity and cytotoxic activity of an endophytic fungus, namely Penicilium roseopurpureum 1E4BS1 isolated from Astragalus angustifolius, have been demonstrated. The aim of this study was to obtain secondary metabolites of P. roseopurpureum and to determine their cytotoxic activity. The ethyl acetate extract of the fermentation broth afforded nine metabolites via isolation studies, and the structures were elucidated by spectral methods (1D-, 2D-NMR, and HR-ESI-MS). Five of the metabolites were found to be new anthraquinone-type compounds. Additionally, the known compounds were identified as 11-methoxycurvularin (epimeric mixture), carviolin, 1-O-methylemodin and 11-hydroxycurvularin (diastereoisomeric mixture). Then cytotoxic activities of the metabolites were determined against three cancer (DU145, LnCaP, and PC3) and a non-cancerous (RPWE-1) prostate cell lines. 11-Methoxycurvularin ([Fig. 1]) exhibited cytotoxic activity in a dose-dependent manner, whereas 11-hydroxycurvularin ([Fig. 1]) had cytostatic properties. These results were further evidenced by 7-AAD/Annexin V staining, which showed that 11-methoxycurvularin induced cellular death while 11-hydroxycurvularin did not increase dead cell content compared to control. Lastly, cell cycle analysis demonstrated that compounds exhibited different cell cycle arrest patterns.