A Comparative study on antioxidant and cytotoxic effects of Oscillatoria amphibia and Spirulina platensis C-phycocyanin and crude extracts
Makaleler > Tam Makale · 2013
Yazarlar: Zeliha DEMİREL, Sevde Uslu Hatipoğlu, Ayşe Nalbantsoy, Fethiye Ferda YILMAZ, Burcu Tepedelen Erbaykent, İsmet Gürhan-Deliloğlu, Meltem DALAY
Ege Journal of Fisheries and Aquatic Sciences, Uluslararası, 2013, ss 1-7
DOI: 10.12714/egejfas.2012.29.1.01
Özet
In this study, antioxidant and cytotoxic effects of Oscillatoria amphibia Ege-Macc-14 (Lake Acıgöl-Denizli, Turkey) C-phycocyanin and the crude extract were compared with Spirulina platensis. Since S. platensis is seen as the sole economical source of C-phycocyanin this comparison study may provide novel information for investigating new sources of C-PC and besides new promising cytotoxic compounds. Both species are cultivated under laboratory conditions and both the crude extracts and the C-PCs were obtained. Since O. amphibia has the possibility to produce toxic compounds, in order to figure out the toxicity of the biomass, the aqueous crude extracts of O. amphibia were tested against the brine shrimp directly. The aqueous extracts were found as non-toxic even at the highest concentrations (10 mg.ml-1). Human hepatocellular carcinoma (HEP-3B) cell line was used to investigate the cytotoxic effect of the extracts. The determined IC50 values of S. platensis C-PC, O. amphibia C-PC were 13.27 μg/ml, 4.19 μg/ml respectively and for S. platensis crude extract and O. amphibia crude extract the values were 16.34 μg/ml and 12.94 μg/ml, respectively. The potential antioxidant activity of the crude extracts and the pure C-PCs were evaluated by DPPH free radical, hydroxyl radical scavenging activity assays. As a result, although the pure C-PC of both cyanobacteria showed cytotoxic and antioxidant activities, the highest activity levels were obtained from O. amphibia extracts.