Lutein Protects Ischemic Skin Flaps via Antioxidant and Anti-Inflammatory Mechanisms in a Rat Model of Ischemia-Reperfusion Injury
Makaleler > Tam Makale · 2025
Yazarlar: ÖVÜNÇ AKDEMİR, Atilla Eyüpoğlu, Emel Öykü ÇETİN UYANIKGİL, Yiğit UYANIKGİL
Journal of Investigative Surgery, SCI İndekslerine Giren Dergi, Uluslararası, Wos, 2025, cilt 38, ss 2528341-2528341
DOI: 10.1080/08941939.2025.2528341
Özet
BACKGROUND: Ischemia-reperfusion injury is a well-recognized challenge in reconstructive flap surgery, often leading to partial or total tissue necrosis. In this experimental study, we aimed to evaluate the protective effects of lutein-a non-provitamin A carotenoid known for its antioxidant and anti-inflammatory actions-against ischemia-reperfusion -induced damage in a rat epigastric flap model. METHODS: Sixteen Sprague-Dawley rats were randomized to receive either intraperitoneal lutein (0.5 mg/kg) or saline prior to inducing 10 h of ischemia. Flap viability was assessed macroscopically on postoperative day 10, and biochemical and histopathological analyses were conducted to explore underlying mechanisms. RESULTS: < 0.01), suggesting reduced oxidative stress and improved vascular function. Histological examination revealed less necrosis, edema, and neutrophil infiltration in the Lutein group, alongside enhanced fibroblast activity, collagen deposition, and neovascularization. Additionally, increased epidermal thickness and a notable rise in lymphocyte infiltration indicated the potential modulation of the adaptive immune response during repair. CONCLUSION: Taken together, our findings suggest that lutein exerts a multifaceted protective effect on ischemic flap tissue and may serve as a useful adjunct in reconstructive surgery, particularly in settings with high risk of ischemia-reperfusion injury. Given its safety and supplement status, these preclinical findings support further exploration in human studies.