Nicotinamide adenine dinucleotide phosphate-diaphorase and c-fos expression in spinal cord neurons following noxious stimulation
Makaleler > Tam Makale · 2008
Yazarlar: Taner DAĞCI, Gulgun Kayalioglu, Burcu Balkan, Berrin E. Okur
Neurosciences, Uluslararası, Wos, 2008, cilt 13, ss 109-112
Özet
OBJECTIVE: To investigate nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) and fos expression in spinal cord dorsal horn neurons following noxious peripheral stimulation. METHODS: Expression of the immediate-early gene c-fos and nitric oxide containing neurons one hour after unilateral formalin injection to the dorsal hind paw was investigated in rat lumbar spinal cord, using fos immunohistochemistry and NADPH-d histochemical techniques. The experiments were performed in 2004 and 2006 at Ege University Center for Brain Research in Izmir, Turkey. RESULTS: In 10 adult male Sprague-Dawley rats, an increase in fos-immunoreactive neurons was observed ipsilateral, and NADPH-d positive neurons equally ipsi- and contralateral to the formalin injection site. Approximately 20% of fos-immunoreactive neurons were NADPH-d positive ipsilateral to the formalin injection, whereas no double labeling was observed in the contralateral side. Also, a close relation of NADPH-d positive processes with fos-immunoreactive nuclei were also observed. CONCLUSION: The results of this study support the hypothesis that nitric oxide synthase blocking agents may serve as a possible alternative in treatment of hyperalgesia following inflammation and peripheral nerve injury.