The role of specific site proteins involved in disease Resistance to Bipolaris (Helminthosporium) sacchari Helminthosporoside toxin
Articles > Article · 1994
Authors: Necip TOSUN
ANADOLU Ege Tarımsal Araştırma Enstitüsü Dergisi, Other Refereed National Journals, National, TR DİZİN (ULAKBİM), 1994, vol 4, pp 103-111
Summary
<p class="MsoBodyText" style="margin-top:0cm;margin-right:15.8pt;margin-bottom: 0cm;margin-left:6.65pt;margin-bottom:.0001pt;text-align:justify;text-justify: inter-ideograph;text-indent:35.3pt;line-height:95%;punctuation-wrap:simple; mso-line-break-override:restrictions"><i style="text-indent: 35.3pt;"><span style="font-size:11.0pt;line-height:95%">Bipolaris sacchari</span></i><span style="text-indent: 35.3pt; font-size: 11pt; line-height: 95%;"> is one of the most important diseases of sugarcane. The helmintho<span style="letter-spacing: -.2pt">sporoside</span><span style="letter-spacing:-.4pt"> </span>(HS) toxin is produced by the pathogen. </span><span style="text-indent: 35.3pt; font-size: 11pt; line-height: 95%;">A high correlation exists between toxin production and pathogenicity. The toxin is considered host specific. The techniques involved in the study of toxin activity include the determination of sensitivity of tissue by electrolyte leakage preparation of radioactive labelled HS toxin and toxin purification, membrane and protein preparations, equilibrium dialysis with scintillation counts of <sup>14</sup>C-HS, and lipid preparation. <span style="letter-spacing:-.75pt">Toxin </span>binding proteins have been found in membranes of non-hosts. Toxin activity is thought to occur on the plasmalemma, where it perturbs the membranes, releasing bound ATPase which lyses cells. A major protein, able to bind the toxin, is present on the plasmalemma, external to the cytoplasm. However, presence of other membrane components is apparently needed for toxin binding and symptom development.</span></p> <p class="MsoBodyText" style="margin-top:0cm;margin-right:15.1pt;margin-bottom: 0cm;margin-left:7.75pt;margin-bottom:.0001pt;punctuation-wrap:simple; mso-line-break-override:restrictions"><b><span style="font-size:11.0pt">Keywords: </span></b><i><span style="font-size:11.0pt">Bipolaris sacchari</span></i><span style="font-size:11.0pt;mso-bidi-font-style:italic">,<b> </b>helmintho<span style="letter-spacing:-.2pt">sporoside</span> toxin, specific side proteins.<o:p></o:p></span></p>