ISOLATION AND IDENTIFICATION OF COPPER AND NICKEL TOLERANT ASCOMYCETOUS YEASTS IN TURKEY
Makaleler > Tam Makale · 2011
Yazarlar: Orcun GUNGOR, FÜSUN BAHRİYE UÇAR, Harun YALÇIN, Cengiz ÇORBACI, Onur AKPINAR
FRESENIUS ENVIRONMENTAL BULLETIN, SCI Expanded İndekslerine Giren Dergi, Uluslararası, 2011, cilt 20, ss 190-197
Özet
Twenty yeast strains were isolated from environments containing different heavy metals and all of these isolates were identified by phenotypic tests. Sixteen isolates detected to be higher in metal tolerance were also identified using the genotypic test (5,8S ITS PCR). Moreover, PCR sequence analysis fragments, which were obtained from ITS-PCR, were accomplished. The phenotypically identified isolates were eight different species belonging to six genera and including four isolates which were not identified by genotypic identification. According to the genotypic identification, the most widely available species was Yarrowia lipolytica (62.5%). Other identified genera were Pichia, Candida and Galactomyces. All the similarities identified as result of sequence analysis were > 90% with the NCBI GenBank. Two strains (strain numbers 14 and 19) were found to be highest tolerant to Cu2+ and Ni2+, respectively, and these results were correlated with findings of the dry weight.