Multiple Mechanisms Synergistically Induce Pseudomonas Aeruginosa Multiple Drug Resistance
Makaleler > Tam Makale · 2023
Yazarlar: Pei Dai, Fangyan Jiao, Lulu Yang, Ousman Bajinka, Khalid A. Abdelhalim, Guojun Wu, Yurong Tan
Microbiology Research, Uluslararası, Scopus, 2023, cilt 14, ss 627-634
DOI: 10.3390/microbiolres14020044
Özet
The aim of this study was to understand the molecular epidemiological characteristics and drug resistance mechanism of carbapenem-resistant Pseudomonas aeruginosa (CRPA) and to provide a theoretical basis for the prevention and treatment of CRPA infection in hospitals. A total of 34 CRPA strains were isolated, and resistance to 13 commonly used antibiotics was detected using the TDR-300B Plus VitEK-2 compact automatic bacterial identification instrument. Then, carbapenemase production was detected using the Carbe NP test. RT-qPCR was used to detect the expression of efflux pump MexA and outer membrane protein OprD, and PCR amplification and sequence analysis were used to detect class Ⅰ integrons carried by drug resistance genes. Our results showed that of the 34 CRPAs, 22 were multi-drug resistant (MDR), and five were extensively drug-resistant (XDR). Sequencing analysis showed that class Ⅰ integron mainly carried aminoglycosides or quinolones resistance genes. Multiple mechanisms play important roles in the formation and development of MDR or XDR.