PERSIMMON (DIOSPYROS KAKI L.) AND JOHNSONGRASS [SORGHUM HALEPENSE (L.) PERS.] ARE NEW NATURAL HOSTS OF PEACH LATENT MOSAIC VIROID
Makaleler > Tam Makale · 2021
Yazarlar: H.D. OKSAL, S. AYDIN, M. BARAN, Ceren EMİR, A. KARANFIL, O. BOZDOĞAN, H.M. SIPAHIOGLU
Applied Ecology and Environmental Research, Uluslararası, Scopus, 2021, cilt 19, ss 4629-4639
DOI: 10.15666/aeer/1906_46294639
Özet
Peach latent mosaic viroid (PLMVd) naturally infects stone fruits worldwide.Here, we report the first detection of PLMVd in persimmon (Diospyros kaki L.) and a weed Johnsongrass [Sorghum halepense (L.) Pers.].Samples corresponding to 12 persimmon specimens and weeds nearby the persimmon trees were collected from a germplasm collection plot in Malatya (Turkey).Total RNAs were isolated using a silica-based method and the complete viroid genome was amplified by reverse transcriptase polymerase chain reaction (RT-PCR).From these samples, PLMVd was detected in 7 of the 12 persimmons and in Johnsongrass revealing 8 new sequence variants.Multiple alignment and phylogenetic analyses revealed that identified persimmon and Johnsongrass isolates clustered only with PLMVd-walnut isolates previously identified from same locality.The nucleotide sequences of PLMVd persimmon and Johnsongrass isolates showed 96.71-99.11%similarity with the PLMVd isolates detected in different fruit crops in the world.A single specific mutation identified in two PLMVd persimmon variants (-TH2 and -TH10) effectively changed the predicted secondary structure of the agent.The identification and the genetic analyses of PLMVd variants in persimmon and Johnsongrass confirm that the agent is a ubiquitous and genetically variable viroid that infects many cultivated fruit crops and weeds worldwide.