Induction of Adventitious Bulb Regeneration via Leaf Explants of Muscari neglectum
Makaleler > Tam Makale · 2025
Yazarlar: AYDIN, Muhammet Anıl, Gülsüm ÖZTÜRK
MAS Journal of Applied Sciences, Uluslararası, 2025
Özet
In this study, shoot organogenesis and micropropagation were derived from in vitro from leaf explants of Muscari neglectum. Leaf explants were cultured in Murashige and Skoog (MS) medium containing TDZ (Thidiazuron) and BA (Benzyladenine) at concentrations of 0.1-0.5 mg L-1 combination with 0.25 mg L-1 NAA (α-naphthalene acetic acid) in cytokinin medium for adventitious bulblet regeneration. The highest means were obtained in 0.5 mg L-1 TDZ + 0.25 mg L-1 NAA in terms of shoot number (23.0), shoot length (1.9), root number (1.0) and root length (1.1 cm). The shoots developed from leaf explants were sub cultured in rooting. The media containing 1.0 mg L-1 indole-3-butyric acid (IBA) had the highest mean for plantlets number (3.0), plantlets length (6.3 cm), root number (5.0) and root length (2.0 cm). Then regenerated plantlets from in vitro were acclimatized under ex vitro in the greenhouse.