Biodistribution of technetium-99m doxycycline hyclate
Articles > Article · 2010
Authors: Derya İLEM ÖZDEMİR, Makbule Asikoglu, Hayal Özkiliç
Fabad Journal of Pharmaceutical Sciences, Journal Indexed in SCI Expanded, International, Scopus, 2010, vol 35, pp 185-189
Summary
The aim of the present study is to evaluate doxycycline hyclate biodistribution by gamma scintigraphy in inflamed rats. The Technetium-99m (99mTc) eluted was used for labeling of doxycycline hyclate. 99mTc-doxycycline hyclate was prepared with a radiochemical yield greater than 90%, adding 99mTc to doxycycline hyclate in the presence of stannous tartarate and ascorbic acid. Scintigraphic studies in rats were carried out using experimentally induced inflammation in the left thigh muscle using turpentine oil. Static images were acquired by a gamma camera in different time intervals for 6 hours. For quantitative evaluation of 99mTc-doxycycline hyclate uptake, the regions of interest were drawn around and uptakes were calculated as counts per pixel. According to in vivo studies, the accumulation of 99mTc-doxycycline hyclate in the inflamed muscle was found higher than the control muscle.