A Histological and Histochemical Investigation on Oocyte Development in Gryllus bimaculatus (Orthoptera: Gryllidae)
Makaleler > Tam Makale · 2016
Yazarlar: Özlem ÇAKICI
Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, Uluslararası, 2016, cilt 19, ss 256-260
Özet
This study investigated the oocyte development in the ovary of two spotted black cricket, Gryllus bimaculatus. Oocytes mature through three developmental stages. These stages include (i) previtellogenic, (ii) vitellogenic and (iii) maturation. In previtellogenic stage, oocytes are encircled by squamous follicle cells. In vitellogenic stage, yolk granules within the oocyte were firstly observed just beneath the oolemma. As vitellogenesis proceeds, yolk granules cover the whole oocyte. In addition, small sized lipid droplets were firstly seen under the yolk granules, then spreads to oocyte. This time, cubic and binucleated follicle epithelial cells encircled the oocyte. In maturation stage, oocytes are full of large yolk granules. Apart from Hematoxylin& Eosin, Periodic Acid Schiff, Bromophenol Blue and Mallory Trichrome reactions were also demonstrated. According to these three histochemical methods, yolk granules showed positive reactivity. Due to the oosorption, the resorption of oocytes, some atretic oocytes were observed. Apoptosis was also observed in atretic follicles. This study indicated that ovary type of Gryllus bimaculatus is panoistic as reported in other Gryllid species. Key Words: Cricket, ovary, oocyte, oosorption, reproduction, vitellogenesis Gryllus bimaculatus ’ta (Orthoptera: Gryllidae) Oosit Gelisimi Uzerine Histolojik ve Histokimyasal Arastirma OZET: Bu calismada iki noktali kara cekirge, Gryllus bimaculatus turune ait bireylerin ovaryumunda histolojik ve histokimyasal incelemeler yapilmistir. Oositler uc asamada olgunlasir, bunlar: (i) previtellojenik, (ii) vitellojenik ve (iii) olgunluk asamalaridir. Previtellojenik evrede oositler yassi folikul epitel hucreleri tarafindan kusatilmaktadir. Vitellojenik evrede yolk granulleri ilk olarak oolemmanin hemen altinda gozlenir. Vitellogenez ilerledikce oositin icini doldururlar. Ayrica kucuk boyutlu lipid damlalari ilkin bu yolk granullerinin altinda bulunur ve daha sonra oositi kaplar. Bu sefer kubik ve cift nukleuslu folikul epitel hucreleri oositi kusatir. Olgunluk evresinde oositler iri yolk granulleriyle kaplidir. Histolojik preparatlar Hematoksilen Eosin boyamasi disinda Periodic Acid Schiff, Bromophenol Blue ve Mallory Trichrome ile boyanarak incelenmistir. Bu uc histokimyasal yontemle yolk granulleri pozitif reaksiyon gostermistir. Oositlerin resorpsiyonu anlamindaki oosit oosorpsiyonu sebebiyle bazi atretik oositler gozlenmistir. Ayrica, apoptozis bu folikullerde belirlenmistir. Bu calisma Gryllus bimaculatus ovaryum tipinin diger Gryllid turlerinde bildirildigi gibi panoistik tipte oldugunu gostermistir. Anahtar Kelimeler: Cekirge, ovaryum, oosit, ureme, vitellogenez