Biosensing approach for glutathione detection using glutathione reductase and sulfhydryl oxidase bienzymatic system
Makaleler > Tam Makale · 2008
Yazarlar: Suna TİMUR, Dilek ODACI, Ayse DINCER, Figen ZİHNİOĞLU, Azmi TELEFONCU
TALANTA, SCI Expanded İndekslerine Giren Dergi, Uluslararası, 2008, cilt 74, ss 1492-1497
DOI: 10.1016/j.talanta.2007.09.026
Özet
Chitosan membrane with glutathione reductase and sulfhydryl oxidase (SOX) was subsequently integrated onto the surface of spectrographic graphite rods for obtaining a glutathione biosensor. The working principle was based on the monitoring of O-2 consumption that correlates the concentration of glutathione during the enzymatic reaction. A linear relationship between sensor response and concentration was obtained between 0.5 and 2.0 mM for oxidized glutathione (GSSG), and 0.2-1.0 mM for reduced glutathione (GSH) in the presence of 2 mu M nicotinamide adenine dinucleotide phosphate (NADPH) under the optimum working conditions. Also, reduced/oxidized glutathione were separated by HPLC and utility of bienzymatic system was investigated as an electrochemical detector for the analysis of these compounds. All data were given as a comparison of two systems: biosensor and diode array detector (DAD). (C) 2007 Elsevier B.V. All rights reserved.