Hydrophobic nano-carrier for lysozyme adsorption
Makaleler > Tam Makale · 2016
Yazarlar: Canan ALTUNBAS, Fulden Zeynep URAL, Murat Uygun, Nesibe AVCIBAŞI, Ugur Avcibasi, Deniz Aktas Uygun, Sinan AKGÖL
BULLETIN OF MATERIALS SCIENCE, SCI Expanded İndekslerine Giren Dergi, Uluslararası, 2016, cilt 39, ss 353-359
DOI: 10.1007/s12034-016-1177-z
Özet
In this work, poly(HEMA-APH) nanoparticles were synthesized by surfactant-free emulsion polymerization technique. Magnetic behaviour was introduced by simple addition of Fe3O4 into the polymerization medium. Characterization of the nanoparticle was carried out by FTIR, ESR, SEM, AFM and EDX analyses. These synthesized magnetic nanoparticles were used for adsorption of lysozyme. For this purpose, adsorption conditions were optimized and maximum lysozyme binding capacity was found to be 278.8 mg g(-1) polymer in pH 7.0 phosphate buffer at 25(a similar to)C. Desorption and reusability properties of the nanoparticles were investigated and lysozyme adsorption efficiency did not change significantly at the end of the 10 successive reuses.