Controlled release of anticancer drug Paclitaxel using nano-structured amphiphilic star-hyperbranched block copolymers
Makaleler > Tam Makale · 2015
Yazarlar: CANER GEYİK, Mustafa Ciftci, Bilal DEMIR, Bahar GULER, A. Burak OZKAYA, Zinar Pınar GÜMÜŞ, F. Baris BARLAS, Dilek ODACI, Hakan COŞKUNOL, Suna TİMUR, Yusuf Yagci
POLYMER CHEMISTRY, SCI Expanded İndekslerine Giren Dergi, Uluslararası, 2015, cilt 6, ss 5470-5477
DOI: 10.1039/c5py00780a
Özet
In the present study, two amphiphilic star-hyperbranched copolymers based on poly(methyl methacrylate)-b-poly(2-hydroxyethyl methacrylate) (PMMA-b-PHEMA), with different hydrophilic PHEMA segment contents (PMMA-b-PHEMA-1, and PMMA-b-PHEMA-2), were synthesized, and their drug loading and release profiles were examined using Paclitaxel (PTX) as a model drug. The drug loading capacities and encapsulation efficiencies were found to be similar in both polymers. The encapsulation efficiencies were found to be prominent at 98% and 98.5% for PMMA-b-PHEMA-1 and PMMA-b-PHEMA-2, respectively. On the other hand, the drug release behaviors varied in favor of the block copolymer comprising shorter PHEMA chains (PMMA-b-PHEMA-1). Additionally, to assess the biological effects of PTX-loaded polymers, human non-small cell lung carcinoma (A549) cells were used. Cell viability and cell cycle analysis showed that both polymers were non-toxic to cells. The cytotoxic effect of PTX-loaded PMMA-b-PHEMA-1 on A 549 cells was greater (66.49% cell viability at 5.0 ng mL(-1) PTX) than that of PMMA-b-PHEMA-2 (72.47% cell viability at 5.0 ng mL(-1) PTX), consistent with the drug release experiments.