Effects of carbon dioxide insufflation on anastomosis remodeling at a carotid artery site in rabbits
Makaleler > Tam Makale · 2018
Yazarlar: Tugra Gencpinar, Gökmen Akkaya, Çağatay Bilen, Pinar Akokay, Osman Yilmaz, Hudai Çatalyürek
Polish Journal of Cardio-Thoracic Surgery, Uluslararası, Scopus, 2018, cilt 15, ss 170-175
Özet
Introduction: Use of carbon dioxide (CO 2 ) insufflation (CDI) on the surgical field during heart surgery has become widespread, and in some units routine.Aim: To assess the effects of CDI on endothelial dysfunction in a carotid artery model in rabbits.Material and methods: Twelve randomly selected rabbits were divided into two groups.Right carotid arteries of the animals were transected and sutured with running suture technique.Then, 1 l/min CO 2 insufflation was initiated with a 45° angle.In the control group, the anastomotic field was irrigated with 0.1 ml/s flow of 0.9% saline.At day 28, the carotid artery segments were removed and prepared for histological specimens.Results: In the cross-sections of the control group vessel samples, thickening of the tunica intima was observed.Scoring the quantity of endothelial nitric oxide synthase (e-NOS) and α-smooth muscle actin (α-SMA) positive staining revealed a nonsignificant difference between the experimental groups (p = 0.07).In the CO 2 group, the intimal hyperplasia (p = 0.2) and the thickness of the tunica media (p = 0.2) were indistinguishable when compared to the control group.The mean luminal diameters and luminal areas of the experimental groups were all evaluated by histomorphometry and a significant differences was found between luminal areas (p = 0.016).On the other hand, no significant difference was found between mean luminal diameters (p = 0.055).Conclusions: Our study indicated that CDI can affect endothelial cell damage and the mean luminal diameters.