Mannose based polymeric nanoparticles for lectin separation
Makaleler > Tam Makale · 2018
Yazarlar: Esra Feyzioğlu Demir, Nevra ÖZTÜRK ATAY, Meryem Koruyucu, Gökhan Kök, Yeşim Salman, Sinan Akgöl
Separation Science and Technology, Uluslararası, Wos, 2018, cilt 53, ss 2365-2375
DOI: 10.1080/01496395.2018.1452943
Özet
The aim of this work is to synthesize the original, new polymeric nanoparticles for concanavalin A (Con A) purification. Nanoparticles were synthesized by surfactant free emulsion polymerization. In the polymerization prosedure, 1-O-(2′-hydroxy-3′-acryloyloxypropyl)-2,3:5,6-di-O-isopropylidene-α-D-mannofuranose (Man-OPA) was used as co-monomer and 2-hydroxyethylmethacrylate (HEMA) was used as a monomer. Man-OPA was characterized by Fourier Transform Infrared Spectroscopy (FTIR), nuclear magnetic resonance and elemental analysis techniques. Poly(HEMA-Man-OPA) nanoparticles were characterized by scanning electron microscopy, FTIR and Zeta Sizer. In adsorption‒desorption experiments, maximum Con A adsorption capacity of poly(HEMA-Man-OPA) nanoparticles was found 630.6 mg/g nanoparticle (pH 7.5, 1.0 mg/mL). Adsorption‒desorption experiments were repeated in four times. According to results, these nanoparticles could be used several times without significant decrease in Con A adsorption capacity.