Detection of Reactive Oxygen and Nitrogen Species by Electron Paramagnetic Resonance (EPR) Technique
Makaleler > Tam Makale · 2017
Yazarlar: Sibel Suzen, Hande GÜRER ORHAN, Luciano SASO
Molecules, SCI Expanded İndekslerine Giren Dergi, Uluslararası, Wos, 2017, cilt 22, ss 181-181
DOI: 10.3390/molecules22010181
Özet
During the last decade there has been growing interest in physical-chemical oxidation processes and the behavior of free radicals in living systems. Radicals are known as intermediate species in a variety of biochemical reactions. Numerous techniques, assays and biomarkers have been used to measure reactive oxygen and nitrogen species (ROS and RNS), and to examine oxidative stress. However, many of these assays are not entirely satisfactory or are used inappropriately. The purpose of this chapter is to review current EPR (Electron Paramagnetic Resonance) spectroscopy methods for measuring ROS, RNS, and their secondary products, and to discuss the strengths and limitations of specific methodological approaches.